5400 Authentication failed Windows 10 clients

[InfraISE2020]

Hi,

We have recently come across an issue where our Windows clients are getting prompted to enter a username/password when connecting to our corporate network. Nothing has been changed on our environment and its been working fine until a few weeks ago. The only change is users have been upgraded to Windows 10 1909, nothing has changed on ISE. 

I have looked through the logs and we're getting an event "5400 Authentication Failed" "11514 Unexpectedly received empty TLS message; treating as a rejection by the client", screenshot of the error attached.

Has anyone else come across this issue and have you been able to resolve it. 

Thanks in advance. 

Ashley

[Sheraz.Salim]

This seem to be a certificate issue. check on ISE certificate tab if EAP authentication certificate is expired. if it down you need to generate a new CSR on ISE and get this signed by your CA. once Signed from your CA upload the cert on ISE. This will fix the issue.